从重组百科病毒感染的昆虫细胞中组装和释放HIV-1前体Pr55gag病毒样颗粒
D Gheysen1, E Jacobs, F de Foresta
1Department of Molecular and Cellular Biology, SmithKline Biologicals, Rixensart, Belgium.
Cell
|October 6, 1989
概括
在昆虫细胞中,复合百科病毒的表达模仿了HIV-1在血膜上形成的Gag粒子. 基化对于芽至关重要,而细胞质/核积累发生在没有它的情况下.
科学领域:
- 病毒学 病毒学
- 分子生物学分子生物学
- 细胞生物学 细胞生物学
背景情况:
- 人类免疫缺陷病毒1型 (HIV-1) 前体 (Pr55gag) 对于病毒颗粒的组装和成熟至关重要.
- 了解逆转录病毒形态发生的分子机制对于开发抗病毒策略至关重要.
研究的目的:
- 通过在昆虫细胞中使用异质表达来研究HIV-1 Gag前体处理和myristoylation在粒子形成中的作用.
- 阐明涉及逆转录病毒粒子形态发生的分子事件.
主要方法:
- 在重组百科病毒感染的昆虫细胞中,HIV-1 Gag前体的表达.
- 通过基因操纵 (例如,N-终端糖氨酸删除,C-终端删除) 来分析 Gag 颗粒的组装,准和处理.
主要成果:
- 艾滋病毒-1 Gag前体向血,形成100-120纳米粒子,模仿未成熟的艾滋病毒粒子芽.
- 化N端甘氨酸对于粒子芽是必不可少的; 它的缺失导致细胞质和核积累.
- 删除C终端 (p16) 取消了粒子聚合,但不会取消膜准/排泄.
- 与多基因共同表达增强了Gag处理,并减少了颗粒的形成.
结论:
- 昆虫细胞中HIV-1 Gag的异质表达为研究逆病毒粒子形态发生提供了有价值的模型.
- 基化在芽过程中起着至关重要的作用,而非基化可能在病毒生命周期中具有其他功能.
- 前体的特定领域对于粒子组装的不同步骤至关重要.
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