相关实验视频

Updated: Apr 16, 2026

Peptide-based Identification of Functional Motifs and their Binding Partners

Peptide-based Identification of Functional Motifs and their Binding Partners

Published on: June 30, 2013

使用微阵列检测预-miRNA-155结合的诱导性活动

Jaeyoung Pai¹, Soonsil Hyun², Ji Young Hyun¹

¹National Creative Research Center for Biofunctional Molecules, Department of Chemistry, Yonsei University , Seoul 03722, Korea.

Journal of the American Chemical Society

|January 16, 2016

概括

研究人员发现了两种抑制微RNA-155 (miRNA-155) 处理的基因,这是癌症中的关键基因. 这些抑制剂通过阻断Dicer活性和诱导细胞亡来促进癌细胞死亡.

科学领域:

分子生物学
癌症学
生物化学

背景情况:

在各种人类癌症中,MicroRNA-155 (miRNA-155) 经常过度表达,通过抑制亡,它起到强大的瘤基因作用.
了解miRNA-155的调节机制对于开发向癌症疗法至关重要.

研究的目的:

确定可以抑制前微RNA-155 (前miRNA-155) 功能的特定.
研究这些抑制剂在促进癌细胞死亡方面的治疗潜力.

主要方法:

基微阵列用于选与前miRNA-155结合的基.
进行了体外Dicer抑制测定和基于细胞的实验,以评估已识别的的功能作用.
用核磁共振 (NMR) 和分子建模研究来阐明结合机制.

主要成果:

鉴定出两种可以有效抑制Dicer介导的预miRNA-155转化为成熟miRNA-155的.
这些抑制剂增强了miRNA-155基因的表达,并通过依赖酶的途径诱导癌细胞的细胞死亡.
结构研究表明,与前miRNA-155的上部突起和顶端茎环区域结合,阻碍了Dicer处理.

结论:

发现了针对miRNA-155前处理的新型抑制剂.
通过诱导亡,这些具有治疗癌症的潜力.

更多相关视频

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Identifying Protein-protein Interaction Sites Using Peptide Arrays

Published on: November 18, 2014

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Published on: November 6, 2021

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...