相关实验视频

Updated: Mar 26, 2026

Production and Targeting of Monovalent Quantum Dots

Production and Targeting of Monovalent Quantum Dots

Published on: October 23, 2014

超小的磁性工程Ag2Se量子点用于即时高效标记和全身高分辨率多模式实时跟踪细胞衍生微小

Jing-Ya Zhao¹, Gang Chen^1,2, Yi-Ping Gu¹

¹Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, State Key Laboratory of Virology, The Institute for Advanced Studies, and Wuhan Institute of Biotechnology, Wuhan University , Wuhan, 430072, P. R. China.

Journal of the American Chemical Society

|January 26, 2016

概括

研究人员开发了超小的Mn-magnetofunctionalized Ag2Se量子点 (Ag2Se@Mn QDs),用于标记细胞衍生微粒 (MVs). 这使得高分辨率,双模式的MVs在体内跟踪治疗应用.

科学领域:

生物医学工程
纳米技术
分子成像

背景情况:

细胞衍生微囊 (MVs) 是具有治疗潜力的自然细胞间载体.
有效的标签策略对于研究和应用MVs in vivo至关重要.
目前的标签方法可能会影响MV的行为或缺乏足够的跟踪能力.

研究的目的:

开发一种新的,高效的MV标签方法.
为了实现高分辨率,双模式的MV的体内追踪.
确保标签不会影响MV的内在行为.

主要方法:

超小型的制造 (约. 1.8 nm) 功能化的Mn-磁体Ag2Se量子点 (Ag2Se@Mn QDs).
对 Ag2Se@Mn QDs 的尺寸,水分散性,NIR光和磁共振 (MR) 放松性进行表征.
使用开发的Ag2Se@Mn QDs进行电穿孔的MV标签.
使用NIR光和MR成像进行标记MV的体内追踪.

主要成果:

超小的Ag2Se@Mn QD具有高的NIR光量子产量和优异的MR纵向放松性.
直接电穿孔有效地将Ag2Se@Mn QD加载到MV中,从而赋予双模式成像能力.
标记方法对于不同来源的MV来说是普遍的,并且不会影响它们的内在行为.
在体内实现了长期的,全身的,高分辨率的MV生物分布跟踪.

结论:

超小的Ag2Se@MnQD提供了一种高效可靠的标签方法.
双模式 (NIR光和MR) 图像使得MV动态的精确追踪.
这种方法有助于微粒的研究和治疗.

更多相关视频

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing MTT

Published on: May 27, 2012

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Published on: September 16, 2022

相关概念视频

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...