相关实验视频

Updated: Mar 26, 2026

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Split-and-pool Synthesis and Characterization of Peptide Tertiary Amide Library

Published on: June 20, 2014

用多种β-氨基酸合成体

Tomoshige Fujino¹, Yuki Goto², Hiroaki Suga²

¹Department of Applied Chemistry, Graduate School of Engineering, Nagoya University , Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan.

Journal of the American Chemical Society

|January 26, 2016

概括

科学家研究了贝塔氨基酸的核糖体翻译, 发现13种是相容的. 然而,多个连续的β-氨基酸是禁止的,但可以与特定的氨基酸插入相结合.

科学领域:

生物化学
分子生物学
合成生物学

背景情况:

几十年的研究已经探索了β-氨基酸与核糖体翻译的兼容性.
关于核糖体接受多种β氨基酸并将多种β氨基酸纳入的能力仍然存在不确定性.

研究的目的:

选贝塔氨基酸以有效的核糖体结合.
使用重新编程的遗传代码合成含有多个β氨基酸的.

主要方法:

使用大肠杆菌复制的无细胞翻译系统.
选各种β-氨基酸的单个和双个合并效率.
为向合成设计的mRNA序列.

主要成果:

确定了13种与核糖体翻译相容的β-氨基酸.
六种β-氨基酸显示出高合效率,而七种显示出中等效率.
禁止连续添加多个β氨基酸,但通过插入Tyr或Ile恢复.

结论:

这项研究证明了使用核糖体机械合成多种不同的β氨基酸的可行性.
特定的氨基酸插入可以克服连续的β-氨基酸结合的限制.
这项工作扩大了工程在各种应用中的潜力.

更多相关视频

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

In Vesiculo Synthesis of Peptide Membrane Precursors for Autonomous Vesicle Growth

Published on: June 28, 2019

An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

An Inexpensive Adaptation of a Commercial Microwave Reactor for Solid Phase Peptide Synthesis

Published on: November 22, 2024

相关概念视频

Ribosomes

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...

Ribosomes

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that synthesize large quantities of protein—such as secretory cells in the human pancreas—can contain millions of ribosomes.
Ribosome Structure and Assembly
Ribosomes are composed of ribosomal RNA (rRNA) and proteins. In eukaryotes, rRNA is transcribed from genes in the nucleolus—a part of the nucleus that specializes in ribosome...

Ribosomes

Ribosomes

Ribosomes

Ribosomes

Leaky Scanning

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA. Marilyn Kozak discovered that the sequence RCCAUGG (where R...

Protein Organization

Protein Organization