2+) 与酸丁乙醇胺结合并增加脂质膜中的氧化
Matthew F Poyton1, Anne M Sendecki1, Xiao Cong2
1Department of Chemistry, Penn State University , University Park, Pennsylvania 16801, United States.
Journal of the American Chemical Society
|January 29, 2016
概括
铜离子 (Cu2+) 与酸丁乙醇胺 (PE) 脂质结合,加速它们的氧化. 这可能解释神经退行性疾病和自闭症中的脂质氧化与高铜水平相关.
科学领域:
- 生物化学
- 生物物理
- 神经科学
背景情况:
- 酸乙醇胺 (PE) 是哺乳动物细胞膜的主要脂质.
- 铜 (Cu2+) 失调与神经退行性疾病和自闭症有关.
研究的目的:
- 研究Cu2+与PE脂质之间的结合相互作用.
- 确定Cu2+对PE脂质氧化的作用.
主要方法:
- 在不同的pH和PE度下测量明显平衡分离常数 (K(DApp)).
- 使用Cu2+和过氧化监测含PE双层中的脂质双键氧化.
主要成果:
- 在生理pH下,Cu2+与PE脂质具有约2μM的K ((DApp) 的双价结合.
- 在pH10.0下,Cu2+与PE的结合亲和度随着PE度的增加而增加 (150nM为70%的PE).
- 在Cu2+和过氧化的存在下,含PE的双层的氧化速度比纯酸胆 (PC) 的双层快8.2倍.
结论:
- 2+与PE脂质的结合增加了它们对氧化的敏感性.
- 在富含PE的膜中增加的脂质氧化可能会导致神经退行性疾病和自闭症相关的高铜水平.
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