相关实验视频

Updated: Mar 26, 2026

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Monitoring Hippo Signaling Pathway Activity Using a Luciferase-based Large Tumor Suppressor LATS Biosensor

Published on: September 13, 2018

托尔受体介导的河马信号控制了多索菲拉的先天免疫力

Bo Liu¹, Yonggang Zheng¹, Feng Yin¹

¹Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

|January 30, 2016

概括

河马的信号控制着组织生长和免疫力. 这项研究揭示了河马 - 约克的信号如何调节Drosophila的仙人掌基因

科学领域:

* 分子生物学
* 免疫学
* 发育生物学

背景情况:

* 由约克犬调节的河马信号通路对于组织生长和平衡至关重要.
它在非发育过程中的作用,如先天免疫力,基本上是未知的.
* Drosophila 的托尔受体通路调解了抗菌反应.

研究的目的:

* 为了研究河马信号在Drosophila天生的免疫反应中的作用.
*阐明Hippo信号调节抗菌产生的机制.
* 在细菌感染期间识别Hippo信号的上游激活器.

主要方法:

在Drosophila脂肪体中对Hippo路径组件和Yorkie进行基因操纵.
* 仙人掌mRNA水平和抗菌表达的定量分析.
* 对Toll-Myd88-Pelle级联及其对STRIPAK PP2A复合物的研究.

主要成果:

* 河马通路中断或约克激活增加了仙人掌mRNA并减少了抗菌.
* 河马信号元件的丧失导致对格拉姆阳性细菌感染的易感性增加.
* 格拉姆阳性细菌通过Toll-Myd88-Pelle通道激活Hippo-Yorkie信号.
* STRIPAK PP2A复合物的Cka亚单元的皮尔介导酸化和降解激活了Hippo信号传递.

结论:

* 河马信号对于Drosophila的先天免疫是必不可少的.
约克直接调节了仙人掌的转录,影响了抗菌的表达.
* 格拉姆阳性细菌通过托尔介导级联激活Hippo信号.
* 这一途径代表了调节先天免疫力的新机制.

更多相关视频

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Long-term In Vivo Tracking of Inflammatory Cell Dynamics Within Drosophila Pupae

Published on: June 14, 2018

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Assessing the Cellular Immune Response of the Fruit Fly, Drosophila melanogaster, Using an In Vivo Phagocytosis Assay

Published on: April 10, 2019

相关概念视频

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...

Hedgehog Signaling Pathway

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

Notch Signaling Pathway

Notch Signaling Pathway

The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...