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在3.7 Å分辨率的酵母U4/U6.U5三snRNP的冷-EM结构

Thi Hoang Duong Nguyen1, Wojciech P Galej1, Xiao-Chen Bai1

  • 1MRC Laboratory of Molecular Biology Francis Crick Avenue Cambridge CB2 0QH UK.

Nature
|February 2, 2016
PubMed
概括

U4/U6.U5三snRNP结合体的冷电子显微镜结构揭示了其组件的原子细节. 这为结合体激活和催化中心的形成提供了新的见解.

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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