大肠杆菌的复制终止蛋白是一种特定于DNA序列的逆酶
G S Khatri1, T MacAllister, P R Sista
1Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710.
Cell
|November 17, 1989
概括
研究人员克隆并表达了tus基因,产生了高度纯净的终结者 (ter) 蛋白. 这种蛋白质作为DNA序列特定的逆酶,阻止DNA在tau位点解开,以终止复制.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 生物化学 生物化学
背景情况:
- DNA复制终止对于基因组稳定性至关重要.
- 大肠杆菌中的tus基因编码了参与这个过程的关键蛋白质.
- 了解复制终止的机制对于细胞生物学来说至关重要.
研究的目的:
- 克隆和表达复制终结蛋白 (ter) 的tus基因.
- 为了净化ter蛋白并描述其在DNA复制终结中的功能.
- 阐明ter蛋白与位DNA相互作用的机制.
主要方法:
- 在大肠杆菌中基因克隆和tus基因的表达.
- 净化ter蛋白质,使其接近同质.
- 使用纯化的ter蛋白和dnaB螺旋酶进行生物化学测试,以评估DNA解抑制.
主要成果:
- 高效表达和高产净化36kDter的蛋白质.
- 证明ter蛋白作为DNA序列特定的逆酶.
- 在tau识别序列上确定ter蛋白对抗酶活性的极性.
结论:
- 蛋白特别与序列结合,并抑制依赖ATP的DNA解.
- 蛋白的极性反酶活性是其在tau位点复制终止中的关键作用.
- 这项研究提供了细菌DNA复制终止的分子机制的见解.
相关概念视频
Replication in Prokaryotes
Overview
Mismatch Repair
Overview
The DNA Helix
Overview
DNA as a Genetic Template
Two structural features of the DNA molecule provide a basis for the mechanisms of heredity: the four nucleotide bases and its double-stranded nature. The Watson-Crick model of double-helical DNA structure, proposed in 1952, drew heavily upon the X-ray crystallography work of researchers Rosalind Franklin and Maurice Wilkins. Watson, Crick, and Wilkins jointly received the Nobel Prize in Physiology or Medicine for their work in 1962. Franklin was, controversially, excluded from the prize for...
Replication in Prokaryotes
DNA replication has three main steps: initiation, elongation, and termination. Replication in prokaryotes begins when initiator proteins bind to the single origin of replication (ori) on the cell's circular chromosome. Replication then proceeds around the entire circle of the chromosome in each direction from the two replication forks, resulting in two DNA molecules.
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Many Proteins Work Together to Replicate the Chromosome
Replication is coordinated and carried out by a host of specialized...
Mismatch Repair
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...


