相关实验视频

Updated: Jun 30, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

在酵母表达的哺乳动物GAP的遗传分析

R Ballester1, T Michaeli, K Ferguson

  • 1Cold Spring Harbor Laboratory, New York 11724.

Cell
|November 17, 1989
PubMed
概括

哺乳动物GTPase激活蛋白 (GAP) 在酵母中通过抑制人类H-rasgly12和补充IRA1突变而起作用. 这表明GAP在调节RAS活动时与酵母IRA1共享生化功能.

更多相关视频

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells07:59

Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Published on: October 5, 2013

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions14:23

A Yeast 2-Hybrid Screen in Batch to Compare Protein Interactions

Published on: June 6, 2018

相关概念视频

Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...