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基于生物灵感的蛋白通道扫描离子导电显微镜 (Bio-SICM) 用于同时导电和特定分子成像

Florika C Macazo¹, Ryan J White¹

¹Department of Chemistry and Biochemistry, University of Maryland Baltimore County , Baltimore, Maryland 21250, United States.

Journal of the American Chemical Society

|February 6, 2016

概括

这项研究引入了生物启发扫描离子导电显微镜 (bio-SICM),这是同时进行孔隙成像和特定分子映射的新技术. 该方法使用蛋白质纳米孔来提高生物分析传感中的化学成像灵敏度.

科学领域:

生物物理
分析化学
纳米技术

背景情况:

用蛋白质纳米孔进行随机单分子检测对于生物分析传感很有价值.
扫描离子导电显微镜 (SICM) 提供地形信息,但缺乏化学特异性.
将蛋白质纳米孔与SICM集成增强了选择性化学成像的能力.

研究的目的:

开发一个生物启发的扫描离子导电显微镜 (bio-SICM) 平台.
实现同时进行孔隙成像和特定分子映射.
证明生物SICM对化学分析的有用性.

主要方法:

使用α-血解素 (αHL) 蛋白通道作为探针.
在单孔玻璃膜上采用光扫描方法.
记录了离子电流以绘制分子存在和孔状特征.

主要成果:

成功地证明了孔和特定分子成像.
使用平均通道电流生成导电图像.
使用原始电流时间痕迹的局部化β-环氧化 (βCD).

结论:

开发的生物SICM平台可以同时对孔和特定分子进行成像.
这种方法为生物分析应用提供了更高的灵敏度和化学选择性.
进一步优化可以将生物SICM用于各种分析需求.

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