胚胎基因振荡器自组织成时空波形
Charisios D Tsiairis1, Alexander Aulehla1
1Developmental Biology Unit, European Molecular Biology Laboratory (EMBL), 69117 Heidelberg, Germany.
Cell
|February 13, 2016
概括
脊椎动物的胚胎发育涉及到前体间皮 (PSM) 细胞组织成结构. 这些细胞自我组织和同步振荡, 重新建立对发育至关重要的波纹.
科学领域:
- 发育生物学
- 细胞动力学
- 系统生物学
背景情况:
- 脊椎动物胚胎中脊椎的前体,起源于前体间皮层 (PSM).
- PSM细胞表现出信号振荡,产生对体生产生必不可少的周期性波纹.
- 在PSM中控制自组织和波纹形成的精确机制仍然不完全理解.
研究的目的:
- 调查前皮层细胞的自我组织能力.
- 为了阐明在PSM中观察到的复杂波纹的起源.
- 了解细胞间相互作用和信号在从无序状态中建立秩序中的作用.
主要方法:
- 采用了体外随机化策略,并结合了前皮质细胞的实时成像.
- 分析了随机PSM细胞的新兴空间排列和振荡动态.
- 研究了诺奇信号在细胞同步和波纹重建中的作用.
主要成果:
- 随机的PSM细胞自发地组织成多个微型新生PSM (ePSM) 结构.
- 这些ePSM结构呈现出有序的宏观空间布局,具有定义的内在长度尺度.
- 细胞以依赖痕信号的方式活跃同步振荡,恢复波状基因活动模式.
- PSM细胞根据周围的细胞线索调整了它们的振荡动态,导致了集体同步.
结论:
- 介质细胞具有自组织和从混乱中产生秩序的内在能力.
- 由Notch信号介导的结合基因振荡器的集体同步是PSM中波形的基础.
- 这些发现突显了细胞组合在发育模式中的新兴特性.
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