表皮三细胞结作为细胞间形状传感器来定位线粒分裂
Floris Bosveld1, Olga Markova1, Boris Guirao1
1Polarity, Division and Morphogenesis Team, Institut Curie, CNRS UMR 3215, INSERM U934, 26 rue d'Ulm, 75248 Paris Cedex 05, France.
Nature
|February 18, 2016
概括
在Drosophila上皮质中通过感知相间细胞形状来导向细胞分裂的三细胞结 (TCJ). 这些结作为极性线索,甚至在细胞分裂过程中指导细胞分裂方向.
科学领域:
- 细胞生物学
- 发育生物学
- 生物物理
背景情况:
- 赫特维格法则描述了细胞分裂方向沿着细胞间长轴,对于组织发育至关重要.
- 表皮细胞的形状受到粘附,应力和极性通路的影响,但在分裂过程中感知这种形状的机制尚不清楚.
- 在线粒分裂过程中,上皮细胞圆形,复杂化了分裂方向的相间形状信息的继承.
研究的目的:
- 研究上皮细胞感知其间相形状以指导细胞分裂的机制.
- 确定参与这种形状感知过程的分子参与者和结构.
- 了解细胞如何在转移到细胞分裂时保持分裂方向.
主要方法:
- 使用Drosophila上皮作为模型系统.
- 研究了三细胞结 (TCJ) 在指导细胞分裂中的作用.
- 使用基因操纵,实时成像和计算模拟.
- 专注于与Dynein相关的蛋白质Mud和Pins/Gαi通路.
主要成果:
- 三细胞结 (TCJ) 定位了拉动星体微管的力量发生器,通过泥蛋白引导细胞分裂.
- 这种定向机制与经典的Pins/Gαi路径独立运作.
- 在线粒循环过程中,TCJs充当空间标志,编码细胞间形状的异构,以指导分裂.
- TCJs的形状和机械应变传感源于它们在上皮组织中的分布几何.
结论:
- TCJs是Drosophila表皮细胞分裂方向的关键调节者.
- TCJ将组织环境中的几何和机械线索整合到分离方向.
- 除了屏障功能之外,TCJs作为表皮形态和组织机制的关键极性线索.
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