Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

17.9K
The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
17.9K
Affinity and Avidity01:41

Affinity and Avidity

40.1K
Overview
40.1K
Hybridoma Technology01:31

Hybridoma Technology

18.3K
Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation,...
18.3K
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

9.9K
The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
9.9K
Antibody Structure01:10

Antibody Structure

67.3K
Overview
Antibodies, also known as immunoglobulins (Ig), are essential players of the adaptive immune system. These antigen-binding proteins are produced by B cells and make up 20 percent of the total blood plasma by weight. In mammals, antibodies fall into five different classes, which each elicits a different biological response upon antigen binding.
The Y-Shaped Structure of Antibodies Consists of Four Polypeptide Chains
Antibodies consist of four polypeptide chains: two identical heavy...
67.3K
Antibody Structure01:10

Antibody Structure

15.1K
No description available
15.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Single-cell analysis of HIV expression and integration sites reveals robust viral expression across diverse chromatin environments.

Microbiology spectrum·2026
Same author

A screen of chromatin-targeting compounds identifies TAF1 as a novel regulator of HIV latency.

mBio·2026
Same author

Clonal dynamics of germinal center refueling by secondary immunization.

bioRxiv : the preprint server for biology·2026
Same author

Replaying germinal center evolution on a quantified affinity landscape.

Cell·2026
Same author

One-step generation of T-cell receptor knock-in mice in the TCRβ locus.

The EMBO journal·2026
Same author

Affinity-matured B cell responses neutralizing type-I interferons underlie severe viral infections.

Cell·2026

相关实验视频

Updated: Mar 23, 2026

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

19.2K

在生殖中心可视化抗体亲和力成熟

Jeroen M J Tas1, Luka Mesin1, Giulia Pasqual1

  • 1Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA.

Science (New York, N.Y.)
|February 26, 2016
PubMed
概括

生殖中心 (GC) 通过B细胞竞争产生高亲和抗体. 这项研究表明,即使没有强烈的选择,GC也可以在亲和成熟期间保持多样化的B细胞克隆,从而影响疫苗的开发.

更多相关视频

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.6K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 10, 2014

10.7K

相关实验视频

Last Updated: Mar 23, 2026

Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

19.2K
Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity
11:12

Interrogating Individual Autoreactive Germinal Centers by Photoactivation in a Mixed Chimeric Model of Autoimmunity

Published on: April 11, 2019

7.6K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 10, 2014

10.7K

科学领域:

  • 免疫学
  • 分子生物学
  • 病毒学

背景情况:

  • 通过生殖中心 (GC) 内的体位突变,抗体获得高亲和力.
  • B细胞克隆及其突变体之间的竞争增加了平均抗体亲和力.
  • 高亲和细胞选择对GC中的克隆多样性的影响尚不清楚.

研究的目的:

  • 研究B细胞克隆多样性与生殖中心亲和力成熟之间的关系.
  • 要确定有效的亲和力成熟是否需要同质化选择.
  • 探索需要非免疫主导抗体特异性的疫苗策略的影响.

主要方法:

  • 使用多光子显微镜可视化GC中的B细胞动态.
  • 使用测序技术来分析B细胞谱的多样性.
  • 结合成像和测序来追踪克隆进化和选择压力.

主要成果:

  • 几十到几百个不同的B细胞克隆启动每一个GC.
  • 基因组具有高度可变的克隆多样性损失率.
  • 有效的抗体亲和力成熟可以在没有强烈的同质化选择的情况下进行.
  • 多个B细胞克隆可以在同一GC中并行成熟.

结论:

  • 生殖中心B细胞的竞争并不总是导致克隆多样性的大幅丧失.
  • 亲和成熟可以同时发生在多种B细胞克隆中.
  • 研究结果表明,可以设计疫苗策略来产生针对较少主导性表位的抗体,这对于HIV-1和流感等病原体至关重要.