在封装DNA宿主中分离,部分氧化和紧的Ag10集群
Jeffrey T Petty1, Orlin O Sergev1, Mainak Ganguly1
1Department of Chemistry, Furman University , Greenville, South Carolina 29613, United States.
Journal of the American Chemical Society
|March 1, 2016
概括
在DNA中形成光学染色体, 一个高度氧化但金属的Ag10集群通过20核基位与DNA结合,形成核心外结构.
科学领域:
- 纳米材料科学
- 生物物理化学
- 光谱学
背景情况:
- 银团可以作为DNA中的光学染色体.
- 这些染色体表现出不同的电子光谱和发射强度.
- 一个特定的银团表现出类似驼的特性, 转化为不同的发射光体.
研究的目的:
- 描述在单链DNA中形成的特定的银团.
- 为了确定星团的大小,电荷,度和结构.
- 为了阐明复杂的整体结构和DNA中的结合.
主要方法:
- 电喷离子质谱 (ESI-MS) 用于集群大小的确定.
- 用于集群电荷分析的Ag L3边缘X射线吸收近边缘结构 (XANES) 光谱.
- 循环二重化 (CD) 光谱用于奇拉性评估.
- 用于结构研究的Ag K边缘扩展X射线吸收细结构 (EXAFS) 光谱.
主要成果:
- 确定了一个稳定的Ag10集群,在不同的寡核酸中很常见.
- 集群的部分电荷为+6到+6.5,表明显著的氧化.
- 集群性取决于DNA序列和长度,约有20个核基的结合点.
- 在EXAFS分析中,发现核心外结构具有明显的银-银协调环境.
结论:
- 一个Ag10(+6) 集群在20核基DNA结合点内形成.
- 这个复合体有一个紧的,金属的白银核心与外层的白银DNA薄弱地连接在一起.
- 这项研究提供了对集群核心中的银银协调的见解.
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