相关实验视频
Updated: Mar 23, 2026

08:07
Simple Detection of Primary Cilia by Immunofluorescence
Published on: May 15, 2020
12.0K
主要毛不是对有反应的机械传感器
M Delling1, A A Indzhykulian2, X Liu1
1Department of Cardiology, Howard Hughes Medical Institute, Boston Children's Hospital, Boston, Massachusetts 02115, USA.
Nature
|March 24, 2016
概括
初级毛没有通过信号感知机械力量,这挑战了长期以来的假设. 这项研究发现,在各种组织中,乳毛中没有机械诱导的增加,这表明了替代的机械感应机制.
科学领域:
- 细胞生物学 细胞生物学
- 生物物理学的生物物理.
- 发育生物学 发展生物学
背景情况:
- 主要毛是参与感知环境的非运动性细胞突起.
- 主流假设认为,初级毛通过 (Ca2+) 信号检测机械力.
研究的目的:
- 为了研究信号传导在初级乳毛中介机械感应中的作用.
- 实验测试Ca2+响应机械传感器假设对初级眼.
主要方法:
- 开发了一只转基因小鼠 (Arl13b-mCherry-GECO1.2) 用于实时对初级乳毛中的成像.
- 测量了Ca2+对各种细胞类型和组织的液体流动的反应,包括脏上皮细胞,胚胎节点冠状细胞和内耳毛细胞.
主要成果:
- 在生理学或超生理学流体流动条件下,在初级乳毛中没有检测到机械诱导的细胞内的增加.
- 在所有测试的细胞类型和组织中观察到缺乏Ca2+流入.
结论:
- 这项研究驳斥了初级毛通过信号传递感知机械力量的假设.
- 如果发生在初级眼中的机械感应,则必须涉及独立于的替代信号通路.
相关概念视频
Microtubules in Signaling
2.3K
The primary cilium, made up of microtubules, acts as antennae on the cell surfaces for relaying external stimuli into the cells. These fine hair-like structures are present, generally one per cell. These are non-motile cilia in a 9+0 microtubules arrangement, where the central pair of microtubules are absent. The primary cilia arise from the basal body embedded in the cell membrane. Intraflagellar transport (IFT) carries requisite proteins from the cytoplasm to the cilium because the primary...
2.3K
Mechanism of Ciliary Motion
5.7K
The ciliary structures were first seen in 1647 by Antonie Leeuwenhoek while observing the protozoans. In lower organisms, these appendages are responsible for cell movement, while in higher organisms, these appendages help in the movement of the extracellular fluids within the body cavities.
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
The cilia are made up of microtubules in a 9+2 arrangement, with nine microtubule doublet ring bundles, surrounding a pair of central singlet microtubule bundles. The doublet microtubule bundles are...
5.7K
Mechanism of Ciliary Motion
2.6K
2.6K
Mechanically-gated Ion Channels
8.0K
Mechanically-gated ion channels are proteins found in eukaryotic and prokaryotic cell membranes that open in response to mechanical stress. Tension, compression, swelling, and shear stress can alter the conformation of the protein, opening a transmembrane channel that allows the passage of ions for signal transmission. In eukaryotes, mechanically-gated channels are distributed in several regions like the neurons, lungs, skin, bladder, and heart, where they play critical roles in numerous...
8.0K
Mechanically-gated Ion Channels
4.3K
4.3K
Tension Response at Adherens Junctions
4.2K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
4.2K

