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肺结核疾病风险的血液RNA签名:前性队列研究
Daniel E Zak1, Adam Penn-Nicholson2, Thomas J Scriba2
1The Center for Infectious Disease Research, Seattle, WA, USA.
Lancet (London, England)
|March 28, 2016
概括
一个全血16基因的新标记可以预测青少年和成年人的肺结核病发病情况. 这一发现为全球抗击结核病的早期干预策略提供了潜在的途径.
科学领域:
- 基因组学
- 传染病流行病学
- 生物标志物发现
背景情况:
- 结核病 (TB) 仍然是全球的主要健康挑战,需要早期发现方法.
- 对于有效的干预措施来说,识别患有从Mycobacterium结核病感染发展为活跃结核病的个体至关重要.
- 目前的诊断方法往往在晚期发现疾病,从而限制了预防的机会.
研究的目的:
- 评估全血基因表达是否可以识别预测活跃结核病风险的潜在特征.
- 开发和验证一种基于血液的基因表达特征,用于结核风险预测.
主要方法:
- 对感染M.结核的南非青少年 (12-18岁) 的前性队列研究.
- 全血RNA测序以确定风险特征,将进展者与对照者进行比较.
- 在青少年和独立的成年人群中使用多重定量实时PCR (qRT-PCR) 验证签名.
主要成果:
- 从全血RNA测序数据中确定了16个基因的风险特征.
- 在青少年诊断前12个月内,该标志显示出中度敏感性 (66. 1%) 和良好的特异性 (80. 6%).
- 在未接触的青少年样本和独立的南非和冈比亚成年人群中成功验证了该签名,显示出显著的预测值 (p<0.018).
结论:
- 全血基因表达特征可以预期识别患有活跃结核病的个体.
- 这一发现为预防结核病进展提供了有针对性的干预措施.
- 验证的签名对处于风险群体的早期风险评估具有前景.
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