一个长非编码RNA与患乳病的易感性相关
Ainara Castellanos-Rubio1, Nora Fernandez-Jimenez2, Radomir Kratchmarov1
1Department of Microbiology and Immunology, Columbia University, College of Physicians and Surgeons, New York, NY 10032, USA.
Science (New York, N.Y.)
|April 2, 2016
概括
一种新的长非编码RNA (lncRNA), lnc13,抑制炎症基因. 在患有乳病的患者中降低的水平和与疾病相关的变体中受损的结合表明Inc13功能障碍有助于炎症.
科学领域:
- 分子生物学
- 遗传学
- 免疫学
背景情况:
- 长非编码RNAs (lncRNAs) 越来越多地被认为是各种生物过程的关键调节者.
- 乳性疾病是小肠的慢性炎症,
研究的目的:
- 鉴定和描述一种与腹腔疾病相关的新型 lncRNA,
- 阐明Inc13在控制炎症基因表达中的调节机制.
- 调查Inc13功能障碍在乳病的产生中的作用.
主要方法:
- 对 lnc13 lncRNA 的鉴定和描述.
- 对Inc13与异质核核蛋白D (hnRNPD) 的相互作用进行分析.
- 精肠活检样本中的Inc13表达量的量化
- 评估与腹腔疾病相关的 lnc13 变体对 hnRNPD 的结合效率.
主要成果:
- 鉴定出Lnc13是一种IncRNA,它携带一种与腹腔疾病相关的哈普类型.
- 在同位状态下,Lnc13通过结合hnRNPD抑制特定炎症基因的表达.
- 在刺激时Lnc13水平下降,导致抑制基因的表达增加.
- 在患有腹腔疾病的小肠活检中,Lnc13的表达显著降低.
- 与野生型相比,与乳病相关的 lnc13 变体对 hnRNPD 的结合 afinity 较低.
结论:
- 低调 lnc13 可能有助于在腹腔疾病中观察到的炎症过程.
- 与疾病相关的 lnc13 变体和 hnRNPD 之间的相互作用损伤为单核型多态对乳病的致病作用提供了分子机制.
- 对于治疗相关炎症而言,Lnc13是一个潜在的治疗.
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