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反对基因对应物Upf3a和Upf3b控制无意义介导的RNA衰变

Eleen Y Shum¹, Samantha H Jones¹, Ada Shao¹

¹Department of Reproductive Medicine, School of Medicine, University of California San Diego, La Jolla, CA 92103, USA.

|April 5, 2016

概括

基因复制产生了UPF3A, 这是一种稳定转录的NMD抑制剂. UPF3A的损失会导致NMD超级,从而导致小鼠的发育缺陷.

科学领域:

进化生物学
分子遗传学
发育生物学

背景情况:

基因复制通过创造新的功能来推动进化.
UPF3B基因对于无意中介RNA衰变 (NMD) 是必不可少的.
它的复制品UPF3A的功能不清楚,NMD活动最小.

研究的目的:

调查UPF3A的功能.
确定对抗基因功能的进化机制.
了解UPF3A在发展中的作用.

主要方法:

在体外和体内功能损失实验.
转录稳定性的分析.
研究UPF3A功能的条件淘汰小鼠模型.
胚胎生殖和生殖组的缺陷评估.

主要成果:

UPF3A作为无意义介导RNA衰变 (NMD) 的强有力的抑制剂.
UPF3A通过抑制NMD来稳定数百个转录.
这可能是UPF3A抑制活性的基础.
缺少UPF3A的小鼠表现出高NMD和发育缺陷.

结论:

在发育过程中,UPF3A充当分子静电剂,调节NMD.
由基因重复产生的敌对功能可能是一个快速的进化机制.
在NMD中UPF3A的作用对于正常的胚胎生成和胚胎生成至关重要.

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