在水性介质中控制长度的单离散圆柱状小胞和块状小胞
Ali Nazemi1, Charlotte E Boott1, David J Lunn1
1School of Chemistry, University of Bristol , Bristol BS8 1TS, United Kingdom.
Journal of the American Chemical Society
|April 7, 2016
概括
研究人员开发了一种精确控制圆柱体块共聚物微粒长度的方法,使其能够在水溶液中使用. 这些新型细胞显示为生物医学应用的基因载体的潜力.
科学领域:
- 聚合物化学
- 材料科学
- 纳米技术
背景情况:
- 圆柱体块共聚物微粒对生物医学研究具有前景,但在水溶剂中缺乏维度控制.
- 在深入研究和应用优化方面,精确控制细胞尺寸至关重要.
研究的目的:
- 开发一种在水中合成具有控制长度和狭窄分布的圆柱状细胞的方法.
- 调查新型两三块细胞的自我组装及其作为基因载体的潜力.
主要方法:
- 通过活体结晶驱动的自组装合成用三乙烯糖醇 (TEG) 装饰的聚乙二甲基乙 (PFS-b-PAGE).
- 使用透析将圆柱状小粒分散到水中.
- 准备和研究两性三块组体 (PFS-b-多和PFS-b-PEO-g-TEG) 和它们的等级自组.
主要成果:
- 在水性介质中成功制备了长度从70nm到1.10μm的圆柱状.
- 发现triblock comicelles的等级自我组装取决于疏水区块的长度.
- P2VP的四分化导致了水溶性圆柱形多块漫细胞,能够复杂化DNA.
结论:
- 在水溶液中制造明确的圆柱体块共聚物微粒的强有力的方法.
- 合成了新型水溶性圆柱形多块漫细胞,证明了它们作为有效的基因传递系统的潜力.
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