相关实验视频

Updated: Mar 22, 2026

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Generation of High Quality Chromatin Immunoprecipitation DNA Template for High-throughput Sequencing ChIP-seq

Published on: April 19, 2013

聚合的ChIP-Seq将转录因子与复杂疾病风险的变化联系在一起

Ashley K Tehranchi¹, Marsha Myrthil², Trevor Martin¹

¹Department of Biology, Stanford University, Stanford, CA 94305, USA.

|April 19, 2016

概括

这项研究引入了一种具有成本效益的方法来绘制影响转录因子 (TF) 结合的遗传变异. 这些发现揭示了遗传变异如何影响基因调节和染色体结构,可能解释人类的特征和疾病.

科学领域:

基因组学
分子生物学
表观遗传学

背景情况:

识别改变转录因子 (TF) 结合的遗传变异对于理解基因调节至关重要.
全基因组关联研究 (GWAS) 将遗传变异与疾病联系起来,但分子机制往往不清楚.

研究的目的:

开发和应用一种成本效益高的基于聚合的方法来绘制TF结合的定量特征位置 (QTL).
研究基因变异对TF结合,染色体结构和染色体结构的影响.

主要方法:

开发了一种基于聚合的策略,用于分子特征的定量特征位置 (QTL) 映射.
应用该方法对五个转录因子和一个基因组修饰的cis-acting QTL进行映射.
将成本效益与标准QTL映射技术进行比较.

主要成果:

与标准方法相比,成功地绘制了成千上万的cis-acting QTL,成本降低了25倍.
发现单个基因变异经常影响多个TF的结合,CTCF招募了五个TF.
观察到这些QTLs影响局部染色体和转录,并影响长距离染色体接触.

结论:

遗传变异在塑造染色体结构和基因调节方面发挥着重要作用.
通过这种方法发现的TF结合变异为GWAS涉及的疾病基因提供了候选分子机制.
TF结合的变化可能是人类表型变化的基础.

更多相关视频

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Identifying Transcription Factor Olig2 Genomic Binding Sites in Acutely Purified PDGFRα+ Cells by Low-cell Chromatin Immunoprecipitation Sequencing Analysis

Published on: April 16, 2018

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Promoter Capture Hi-C: High-resolution, Genome-wide Profiling of Promoter Interactions

Published on: June 28, 2018

相关概念视频

General Transcription Factors

General Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Transcription Factors

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...