酸附加分子剂用于酶的ATP反应活性调节
Kou Okuro1, Mizuki Sasaki1, Takuzo Aida1,2
1Department of Chemistry and Biotechnology, School of Engineering, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan.
Journal of the American Chemical Society
|April 19, 2016
概括
新型聚合物GumBAn通过与腺三酸盐 (ATP) 结合起作用,作为酶活性的ATP反应调节剂. 这些聚合物使素等酶失活,但在添加ATP时释放它们,在低度下恢复活性.
科学领域:
- 聚合物化学
- 生物化学
- 酶工程
背景情况:
- 开发适应生物过程的材料至关重要.
- 腺三酸盐 (ATP) 是一个关键的细胞能量分子, 调节许多生物功能.
- 酶活性调节对于各种生化应用和治疗策略至关重要.
研究的目的:
- 合成和表征水溶性线性聚合物 (GumBAn) 使用双功能的悬挂物 (离子和酸).
- 研究GumBAn聚合物作为对ATP反应的酶活性调节剂.
- 通过使用GumBAn和ATP来证明素 (Trp) 的受控失活和重新激活.
主要方法:
- 合成GumBAn聚合物,其中离子 (Gu(+)) 和酸 (BA) 的比例不同.
- 聚合物-离子和酸与腺三酸盐 (ATP) 和蛋白质相互作用的特征.
- 使用素 (Trp) 进行酶活性测定,以在ATP存在或不存在的情况下证明GumBAn的调节,包括在细胞条件下.
主要成果:
- GumBAn聚合物通过Gu(+) 和BA悬挂有效地与ATP结合.
- GumBAn通过杂交成功地禁用了素活性.
- 添加ATP从GumBAn释放了素,在ATP度明显低于之前报告的水平时恢复了水解活性;在细胞条件下也观察到调节.
结论:
- GumBAn聚合物代表了一种新的ATP反应调节剂,用于酶活性.
- (+) 悬挂是蛋白质相互作用的关键,使酶调节成为可能.
- 开发的系统提供了对酶活性的高效和灵敏的控制,在生物系统和治疗中具有潜在的应用.
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