膜透的Mn(III) 复合物用于细胞内标的分子磁共振成像
Ali Barandov1, Benjamin B Bartelle1, Beatriz A Gonzalez1
1Departments of †Biological Engineering, ‡Chemistry, §Brain and Cognitive Sciences, and ⊥Nuclear Science and Engineering, Massachusetts Institute of Technology , 77 Massachusetts Avenue, Cambridge, Massachusetts 02139, United States.
Journal of the American Chemical Society
|April 19, 2016
概括
研究人员开发了新的基于 (Mn(III)) 的对比剂,用于磁共振成像 (MRI). 这些探测器有效地准细胞内空间,使细胞内的分子成像能够得到增强.
科学领域:
- 生物医学成像技术 生物医学成像技术
- 分子成像学分子成像学
- 纳米技术 纳米技术
背景情况:
- 细胞内是至关重要的,但很难用MRI进行成像.
- 标准的MRI对比剂与细胞膜透性作斗争.
- 高分辨率的MRI需要敏感的探针来检测细胞内分析物.
研究的目的:
- 开发新的MRI对比剂,用于有效的细胞内传递和成像.
- 创建一个平台,用于细胞内目标的分子MRI.
- 克服当前MRI对比剂在进入细胞空间方面的局限性.
主要方法:
- 合成的平面四牙酸 (Mn(III) 酸盐具有1,2-二胺基 (PDA) 骨干.
- 研究了Mn(III) -PDA复合物的自发细胞溶液局部化机制.
- 开发了用于细胞积累的具有酶可切割的乙甲基基组的探针变体.
- 利用基质选择性雌激酶表达来进行向细胞标记.
主要成果:
- (III) -PDA复合物显示T1放松性与加多 (Gd (III)) 剂相当.
- 探测器证明了自发地定位到细胞质中.
- 酶可分裂的变体在细胞内有效积累.
- 实现了强大的T1加权MRI增强,表明细胞内检测成功.
结论:
- III-PDA复合体为细胞内点的分子MRI提供了一个可行的策略.
- 这些药物为开发用于细胞内成像的试剂提供了一个多功能平台.
- 这项研究建立了使用新型Mn(III) 探针检测细胞内分析物的先例.
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