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来自Nitrosopumilus maritimus的紫色素,含有开放结合点的单核1型铜中心

Parisa Hosseinzadeh, Shiliang Tian, Nicholas M Marshall

¹Biology Department, Brookhaven National Laboratory , Upton, New York 11973-5000, United States.

Journal of the American Chemical Society

|April 28, 2016

概括

研究人员发现了一种来自Nitrosopumilus maritimus SCM1的新型素Nmar1307. 这种蛋白质具有不寻常的开放型1铜 (T1Cu) 中心与水联体,使NO氧化并扩大已知的cupredoxins的功能.

科学领域:

生物化学
生物物理
古生物生物学

背景情况:

单核铜通常具有和型1铜 (T1Cu) 中心.
这些蛋白质传统上仅作为电子载体而起作用.

研究的目的:

来自古生物Nitrosopumilus maritimusSCM1的一种新型铜素Nmar1307的特征.
研究其独特的T1Cu中心的结构和功能特性.

主要方法:

紫外线电子光谱测定光谱性质.
电子磁共振 (EPR) 光谱分析铜种.
进行X射线晶体学以解析原子结构 (1.6 Å分辨率).

主要成果:

Nmar1307呈现深紫色,在413nm和558nm时具有强烈的紫外线吸收.
经过EPR研究,发现了两种不同的Cu (II) 种,一种是轴性,另一种是形.
X射线结构证实T1Cu中心有两个His,一个Cys和一个轴性水分子.
这种蛋白质具有氧化氧化 (NO) 到酸盐的能力,这归因于其高降解潜力 (354mV与SHE).

结论:

单核铜可以具有可变的结构特征,包括与水的开放结合点.
发现Nmar1307突出了 cupredoxins 超出了简单的电子转移.
这种古老的毒素扩大了这一类蛋白质的功能范围.

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