全球对乙转移酶反的规范
David C Montgomery1, Julie M Garlick1, Rhushikesh A Kulkarni1
1Chemical Biology Laboratory, National Cancer Institute , Frederick, Maryland 21702, United States.
Journal of the American Chemical Society
|May 6, 2016
概括
研究人员开发了一种使用抑制剂功能化树脂的新方法来分析酸转移酶 (KAT) 相互作用. 这项技术揭示了KAT对CoA的不同敏感性,并确定了NAT10作为代谢反的潜在目标.
科学领域:
- 生物化学
- 细胞信号传输
- 蛋白质组学
背景情况:
- 酸乙转移酶 (KAT) 是调节细胞信号通路的关键酶.
- 了解KAT调节对于破译细胞过程和疾病机制至关重要.
- 现有的KAT相互作用分析方法在范围和灵敏度方面存在局限性.
研究的目的:
- 在复杂的生物样本中开发和优化化学蛋白质平台.
- 综合分析代谢物辅酶A (CoA) 对KATs的抑制.
- 通过代谢反调节的新型KAT.
主要方法:
- 使用抑制剂功能化的捕获树脂进行基于亲和性的KATs和相关复合物的丰富.
- 应用于复杂蛋白质的化学蛋白质分析,以确定KAT-连接体相互作用.
- 通过CoA对KAT抑制进行了全面分析.
主要成果:
- 优化的平台成功地分析了更广泛的KAT,KAT复合体和CoA依赖酶.
- 在KAT超级家族中发现了对CoA的敏感性范围.
- 鉴定了1型核转移酶 (NAT10) 作为一种新型的KAT,易受CoA介导的反抑制.
结论:
- 开发的化学蛋白质平台为研究KAT调节提供了强大的工具.
- 这些发现为KAT依赖信号通路的代谢控制提供了新的见解.
- 这种方法可用于评估KAT可逆调节剂的效力和选择性,包括小分子和代谢物.
相关概念视频
Regulation of Expression at Multiple Steps
1.5K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.5K
Allosteric Regulation
64.3K
Allosteric regulation of enzymes occurs when the binding of an effector molecule to a site that is different from the active site causes a change in the enzymatic activity. This alternate site is called an allosteric site, and an enzyme can contain more than one of these sites. Allosteric regulation can either be positive or negative, resulting in an increase or decrease in enzyme activity. Most enzymes that display allosteric regulation are metabolic enzymes involved in the degradation or...
64.3K
Allosteric Regulation
16.1K
16.1K
Feedback Inhibition
58.5K
Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
58.5K
Covalently Linked Protein Regulators
9.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
These groups modify specific amino acids in a protein....
9.9K
Regulation of Metabolism
12.1K
Cellular needs and conditions vary from cell to cell and change within individual cells over time. For example, the required enzymes and energetic demands of stomach cells are different from those of fat storage cells, skin cells, blood cells, and nerve cells. Furthermore, a digestive cell works much harder to process and break down nutrients during the time that closely follows a meal compared with many hours after a meal. As these cellular demands and conditions vary, so do the amounts and...
12.1K


