发育过程中获得的调节体是先天性淋巴细胞功能的基础
Han-Yu Shih1, Giuseppe Sciumè1, Yohei Mikami1
1Lymphocyte Cell Biology Section, Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Cell
|May 10, 2016
概括
天生的淋巴细胞 (ILC) 和T辅助细胞 (Th) 分享调节性DNA元素. 这项研究揭示了这些先天性和适应性免疫细胞之间广泛的共享调节电路,尽管它们的发育途径不同.
科学领域:
- 免疫学
- 分子生物学
- 基因组学
背景情况:
- 天生的淋巴细胞 (ILC) 对于免疫反应至关重要,反映了适应性CD4 ((+) T辅助 (Th) 细胞的功能.
- 了解ILC和Th细胞之间的关系对于理解免疫系统调节至关重要.
研究的目的:
- 通过测量全基因组染色体可访问性来研究ILC子集和Th细胞对应物之间的关系.
- 确定先天性和适应性免疫组之间的共享调节电路.
主要方法:
- 对ILCs和CD4 ((+) T细胞进行了全基因组染色体可访问性分析.
- 分析的重点是靠近效应基因的调节区域.
- 用一种2型感染模型研究Th细胞分化.
主要成果:
- 在激活之前,在发育过程中获得的染色体在效应基因附近是有选择性的.
- 相比之下,原始的CD4 ((+) T细胞在Th细胞分化过程中经历了显著的染色质重塑.
- Th2细胞对ILC2规基体有很大的趋同,表明它们具有共同的调节元素.
结论:
- 在先天性 (ILC) 和适应性 (Th细胞) 免疫区间之间存在广泛的调节电路共享.
- 在ILC中,染色体可访问性模式在很大程度上是在发育过程中建立的,并在激活时保持稳定.
- 免疫细胞的分化涉及显著的染色体重塑,导致调节场景的融合.
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