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基于纸张的免疫测试的质谱:向按需诊断

Suming Chen¹, Qiongqiong Wan¹, Abraham K Badu-Tawiah¹

¹Department of Chemistry and Biochemistry, The Ohio State University , Columbus, Ohio 43210, United States.

Journal of the American Chemical Society

|May 10, 2016

概括

一个新的诊断平台使用纸质质谱仪进行按需测试. 这种方法可以对疟疾和癌症等疾病进行敏感,可中断的检测,

科学领域:

分析化学
生物医学诊断
质谱学

背景情况:

现有的诊断方法 (临床和集中诊断) 在满足患者友好测试和可靠结果的需求方面存在局限性.
需要创新的诊断策略,

研究的目的:

引入一种新的两点分离点诊断策略.
开发一种基于纸质的质谱免疫测试平台,用于可中断和可恢复的按需检测.
展示一种新的触摸纸喷雾方法,用于敏感的芯片上分析物检测.

主要方法:

使用稳定和可裂变的离子探测器作为质量报告器的纸质质谱免疫试验的开发.
采用两点分离技术进行按需分析.
引入触摸纸喷雾方法用于芯片样本的引入和检测.

主要成果:

该平台可实现敏感,可中断,可存储和可恢复的按需检测.
成功检测了Plasmodium falciparum胺蛋白2抗原.
已证实多重和同时检测癌症抗原125和癌胚抗原.

结论:

开发的基于纸质的质谱免疫测试平台为患者友好和可靠的诊断提供了有前途的解决方案.
按需策略和触摸纸喷涂方法提高了灵敏度和成本效益.
这种方法是多用途的,适用于传染病和癌症生物标志物检测.

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