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使用可编程生物分子组件快速,低成本检测寨卡病毒

Keith Pardee¹, Alexander A Green², Melissa K Takahashi³

¹Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON M5S 3M2, Canada.

|May 11, 2016

概括

研究人员开发了一种快速,低成本的纸质传感器来检测寨卡病毒RNA. 这种使用CRISPR/Cas9的合成生物学工具为全球卫生危机提供现场准备的诊断.

科学领域:

生物技术
分子生物学
全球健康

背景情况:

寨卡病毒爆发强调了在受影响地区需要可访问,快速部署的诊断.
现有的诊断方法通常面临成本,复杂性和现场可用性方面的挑战.

研究的目的:

开发一种快速,低成本,无细胞,基于纸张的传感器系统,用于检测寨卡病毒RNA.
为了证明传感器的特异性,灵敏性和现场准备性.

主要方法:

建立了一个设计,组装和验证无细胞纸质传感器的管道.
隔热RNA放大与脚开关RNA传感器相结合用于发现寨卡病毒.
一个基于CRISPR/Cas9的模块被整合到病毒菌株之间进行单基分辨.

主要成果:

检测到临床相关的寨卡病毒序列,对登革热病毒的特异性很高.
该系统使用CRISPR/Cas9进行了单基分辨率.
一个现场准备的样本处理工作流程成功地被证明,包括从的血中检测.

结论:

开发的冷干燥纸质传感器平台解决了在现场部署分子诊断的实际限制.
合成生物学方法可以有效地为像寨卡病毒爆发这样的全球卫生紧急情况创造创新的诊断工具.

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