TMCO1 是一个ER Ca2+) 负载激活的Ca2+) 通道.
Qiao-Chu Wang1, Qiaoxia Zheng1, Haiyan Tan2
1State Key Laboratory of Membrane Biology, Institute of Zoology, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100101, China.
细胞内膜网膜 (ER) 蛋白TMCO1作为负载激活通道 (CLAC),防止ER储量过度填充. 这一发现为细胞平衡和相关的发育障碍提供了洞察力.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物物理学的生物物理.
背景情况:
- 维持内网 (ER) 中的 (Ca2+) 稳态对于细胞信号传递和功能至关重要.
- Ca2+释放激活的Ca2+ (CRAC) 通道补充ER储存,但防止ER Ca2+过载的机制尚不清楚.
研究的目的:
- 研究TMCO1在调节ER Ca2+水平中的作用.
- 将TMCO1描述为一个潜在的Ca2+通道,用于防止ER Ca2+过载.
主要方法:
- 研究了TMCO1在ER Ca2+调节中的功能.
- 研究了对Ca2+水平的反应中TMCO1的寡合化状态.
- 在巨型脂质体中重建TMCO1通道活性.
- 在淘汰赛小鼠模型中检查TMCO1功能.
主要成果:
- TMCO1是一种ER跨膜蛋白,可以防止ER Ca2+储量过度填充.
- TMCO1作为一个Ca2+负载激活的Ca2+通道 (CLAC) 起作用.
- 在ER Ca2+过载时,TMCO1经历可逆四聚变,并在耗尽时分解.
- TMCO1 淘汰赛小鼠表现出 ER Ca2+ 错误处理和脑faciothoracic 发育不良谱的特征.
结论:
- TMCO1作为一个重要的保护机制,防止ER Ca2+过度填充.
- TMCO1 功能障碍与大脑faciothoracic 增生障碍谱系障碍有关.
- TMCO1代表了理解和治疗Ca2+失调障碍的新目标.
更多相关视频
14:18Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber
Published on: February 13, 2012
08:27Expression and Purification of the Human Lipid-sensitive Cation Channel TRPC3 for Structural Determination by Single-particle Cryo-electron Microscopy
Published on: January 7, 2019
相关概念视频
Calmodulin-dependent Signaling
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...
Mechanically-gated Ion Channels
Mechanically-gated Ion Channels
Voltage-gated Ion Channels
Generally, all voltage-gated ion channels have a 'voltage-sensing domain' that spans the lipid bilayer. The charged residues in the sensor move in response to the membrane potential changes that open the channel allowing ions movement. There are several types of...
Voltage-gated Ion Channels
