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自燃硫酸盐可提供触发的H2S供体和分析物的替代光探针

Andrea K Steiger¹, Sibile Pardue², Christopher G Kevil²

¹Department of Chemistry and Biochemistry, Materials Science Institute, Institute of Molecular Biology, University of Oregon , Eugene, Oregon 97403, United States.

Journal of the American Chemical Society

|May 25, 2016

概括

研究人员使用自焚基碳酸盐开发了一种新硫化物 (H2S) 输送系统. 这种创新的化学工具可以控制H2S的释放,并应对生物信号研究的挑战.

科学领域:

化学生物学
生物化学
有机化学

背景情况:

硫化 (H2S) 是一个重要的生物信号分子.
化学工具对于研究中的H2S输送和检测至关重要.
现有的H2S捐赠者和探测器面临着刺激触发释放和分析物消耗等挑战.

研究的目的:

为控制硫化 (H2S) 捐赠制定新策略.
通过特定的刺激来产生H2S捐赠体.
在基于H2S反应的探针中解决分析物的恒温问题.

主要方法:

采用了一种基于自焚基碳酸盐的新型H2S捐赠策略.
碳酸硫化物 (COS) 被释放,并通过碳酸无水酶迅速转化为H2S.
这种化学反应被用来制造易于修改的刺激触发的H2S捐赠体.
这种方法与H2S感应图案相结合,以创建光探针.

主要成果:

一个新的H2S捐赠机制成功建立.
合成了易于修饰的,对刺激有反应的H2S供体.
开发的探针在H2S反应时表现出光反应.
探测器释放中的H2S,保持分析物的平衡.

结论:

甲基酸盐的自焚为H2S捐赠提供了一个多功能平台.
这一策略克服了现有的H2S输送和检测方法的局限性.
开发的探测器为研究H2S的生物作用提供了有前途的工具.

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