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通过直接早期基因的自动化体积分析绘制大脑活动图

Nicolas Renier¹, Eliza L Adams¹, Christoph Kirst²

¹Laboratory of Brain Development and Repair, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA.

|May 31, 2016

概括

研究人员开发了一种新的管道来绘制整个哺乳动物大脑的所有神经元活动. 这种方法使用基因表达分析和先进的成像来分析各种条件下的神经回路.

科学领域:

神经科学
分子生物学
生物成像

背景情况:

在生理和病理状态下理解神经信息处理需要全面的脑活动映射.
目前的方法缺乏对哺乳动物大脑进行完整分析所需的细胞分辨率和全脑覆盖.

研究的目的:

在整个哺乳动物大脑中采集和分析高速的细胞分辨率大脑活动.
在各种实验范式中准确检测,定位和量化神经元活动.

主要方法:

通过免疫染色进行即时早期基因表达的分析.
高速光片光成像用于数据采集.
使用开源软件ClearMap进行自动映射和分析.

主要成果:

通过分析皮醇激活的大脑区域来验证管道.
在活跃探索过程中发现了新的皮质区域.
组合活动映射与轴突追踪以揭示在育儿行为中差异激活的大脑区域.

结论:

开发的管道为全脑细胞分辨率活动绘制提供了广泛适用的方法.
这种方法对于在各种条件和物种中研究神经回路是有价值的,包括那些没有转基因记者的人.

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Brain Imaging

Brain Imaging

Brain imaging technologies provide critical insights into both the structure and function of the human brain, enabling medical professionals and researchers to diagnose, study, and treat neurological disorders or psychiatric disorders more effectively.
These technologies include computerized axial tomography (CAT or CT scans), positron-emission tomography (PET scans), magnetic resonance imaging (MRI), functional magnetic resonance imaging (fMRI), and Transcranial Magnetic Stimulation (TMS).