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相关概念视频

RNA-seq03:21

RNA-seq

12.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

Ribosome Profiling

4.3K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Mar 20, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
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Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

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TT-seq绘制了人类的短暂转录组

Björn Schwalb1, Margaux Michel1, Benedikt Zacher2

  • 1Department of Molecular Biology, Max Planck Institute for Biophysical Chemistry, Am Faßberg 11, 37077 Göttingen, Germany.

Science (New York, N.Y.)
|June 4, 2016
PubMed
概括
此摘要是机器生成的。

暂时转录组测序 (TT-seq) 映射所有RNA类型,揭示短期增强器RNA并识别转录终结位. 这种方法提供了全基因组RNA合成和降解动态的全面视图.

更多相关视频

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

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Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
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Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

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相关实验视频

Last Updated: Mar 20, 2026

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq
09:08

Mapping Genome-wide Accessible Chromatin in Primary Human T Lymphocytes by ATAC-Seq

Published on: November 13, 2017

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Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis
12:44

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

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Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms
05:12

Author Spotlight: AQRNA-seq Role in Mapping Small RNAs and Unraveling Protein Translation Mechanisms

Published on: February 2, 2024

1.5K

科学领域:

  • 基因组学
  • 分子生物学
  • 转录组学

背景情况:

  • 基因组不断产生各种RNA分子,包括稳定和短暂的形式.
  • 了解RNA生产和循环的全谱对于解读基因调节至关重要.

研究的目的:

  • 开发和应用一种新的方法,即短暂转录组测序 (TT-seq),用于对RNA生成单元进行全面的映射.
  • 估计整个基因组的RNA合成和降解速率.
  • 描述过渡性RNA,包括增强性RNA,并确定转录终结点.

主要方法:

  • 开发过渡性转录组测序 (TT-seq) 协议.
  • TT-seq对人类K562细胞的应用.
  • 测序数据的生物信息分析以绘制RNA物种和终结点.

主要成果:

  • TT-seq成功地绘制了稳定的信使RNA,长非编码RNA和各种短暂RNA (增强剂,反意义,促进剂相关).
  • 增强器RNA被描述为缺乏U1动机和显著的二次结构的短寿命分子.
  • 确定了多个转录终结位,与促进RNA聚合酶暂停的DNA动机相关.

结论:

  • TT-seq提供了一种统一的方法来描述整个转录组,包括稳定和短暂的RNA物种.
  • 这项研究为过渡性RNA,特别是增强性RNA的特性和调节提供了新的见解.
  • 识别转录终结点有助于对RNA处理和基因组调节的理解.