一个长非编码RNAlinkRNA-EPS作为一个转录制动器来抑制炎症
Maninjay K Atianand1, Wenqian Hu2, Ansuman T Satpathy3
1Program in Innate Immunity, University of Massachusetts Medical School, Worcester, MA 01605, USA.
Cell
|June 18, 2016
概括
长非编码RNAs (lncRNAs) 调节基因表达. 我们发现了lincRNA-EPS, 这是一种抑制巨细胞炎症基因表达的新型lincRNA,
科学领域:
- 免疫学
- 分子生物学
- 遗传学
背景情况:
- 长跨基因非编码RNAs (lincRNAs) 是基因表达的新兴调节者.
- lncRNAs在免疫细胞功能中的作用在很大程度上是未知的.
- 巨细胞是参与炎症反应的关键免疫细胞.
研究的目的:
- 识别和描述新的免疫调节链接RNA.
- 阐明lincRNA-EPS在巨介导免疫反应中的作用.
- 研究lincRNA-EPS控制基因表达的分子机制.
主要方法:
- 对lincRNA-EPS缺陷的巨细胞进行转录组分析.
- 在巨细胞中获得功能和救援实验.
- 在lincRNA-EPS缺少小鼠的体内研究中,使用了内毒素.
- 染色体免疫沉和RNA结合测试以确定lincRNA的局部化和相互作用.
主要成果:
- 在巨细胞中精确调节,抑制免疫反应基因 (IRG) 的表达.
- 缺乏lincRNA- EPS的小鼠在受到内毒素刺激时会出现炎症和死亡率的增加.
- 影响核细胞的定位并抑制转录.
- 通过特定的动机与异质核核糖核蛋白L (hnRNP L) 相互作用,以调解其抑制功能.
结论:
- 在巨细胞中, lincRNA- EPS 作为炎症基因表达的关键抑制剂.
- 这项研究强调了lincRNAs在调节先天免疫力的重要作用.
- 对于炎症性疾病来说,lincRNA- EPS是一个潜在的治疗点.
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