具有超快切换功能的绿色光蛋白光体模拟器的设计,合成和动力学
Marco Paolino1, Moussa Gueye2, Elisa Pieri1
1Dipartimento di Biotecnologie, Chimica e Farmacia, Università di Siena , Via A. Moro 2, 53100 Siena, Italy.
Journal of the American Chemical Society
|June 21, 2016
概括
在绿色光蛋白的启发下, 研究人员开发了新的离子分子开关. 这些开关表现出快速的轴旋转运动,为现有的阴离子系统提供了补充性质.
科学领域:
- 有机化学
- 摄影化学
- 超分子化学
背景情况:
- 旋转分子开关对于先进的材料和设备至关重要.
- 现有的开关主要是中性或阴性,限制了它们的应用.
- 需要具有互补性质的离子开关.
研究的目的:
- 设计和合成新的离子分子开关.
- 调查它们的E/Z切换功能的机制和动态.
- 为了比较它们的属性与性对应物.
主要方法:
- 计算模型和模拟.
- 实验调查包括瞬态光谱.
- 阳离子p-HBDI染色体模拟的合成
主要成果:
- 离子分子开关的成功准备.
- 在小于皮秒的时间尺度上确认轴旋转运动.
- 在形交叉点附近识别非adiabatic衰变.
结论:
- 可以有效地设计和合成离子分子开关.
- 这些开关表现出快速,可预测的旋转运动.
- 它们为分子设备的现有离子开关提供了补充功能.
更多相关视频
10:31Residue-Specific Exchange of Proline by Proline Analogs in Fluorescent Proteins: How "Molecular Surgery" of the Backbone Affects Folding and Stability
Published on: February 3, 2022
3.5K
11:03How to Quantify the Fraction of Photoactivated Fluorescent Proteins in Bulk and in Live Cells
Published on: January 7, 2019
7.1K
相关概念视频
02:11Reporter Genes
13.7K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
13.7K
01:19Protein Dynamics in Living Cells
2.8K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.8K