葡萄糖皮质激素和孕的特异性作用是由各自激素受体的差异性表达决定的
U Strähle1, M Boshart, G Klock
1Deutsches Krebsforschungszentrum, Institut für Zell- und Tumorbiologie, Heidelberg, FRG.
Nature
|June 22, 1989
概括
激素受体,如葡萄糖皮质激素和孕激素受体,与相同的DNA序列结合. 将孕激素受体引入细胞重新编程基因表达,证明差异性受体表达可以实现类固醇选择性.
科学领域:
- 分子内分泌学分子内分泌学
- 遗传学 是一个遗传学.
- 细胞生物学 细胞生物学
背景情况:
- 葡萄皮质醇和孕激素调节不同的生理过程.
- 葡萄糖皮质体和孕激素的激素受体与相同的DNA序列结合.
- 这些类固醇对选择性基因表达的机制尚不清楚.
研究的目的:
- 研究葡萄糖皮质体和孕激素受体如何实现选择性基因表达,尽管它们与相同的DNA元素结合.
- 为了确定差异性受体表达或对其他因素的依赖是否驱动选择性.
主要方法:
- 利用合成基因的转移实验和体外结合研究.
- 引入一种孕激素受体表达等离子体到一条缺乏内源性孕激素受体但具有葡萄糖皮质体受体的老鼠肝瘤细胞系 (Fto2B-3).
- 评估了由孕激素调节的内源性葡萄糖皮质激素基因的诱导性.
主要成果:
- 孕激素受体在Fto2B-3细胞中的表达使孕激素能够诱导葡萄糖皮质激素调节的基因.
- 证明引入单个转录因子可以重编程细胞反应.
- 提供证据表明,激素受体的差异表达是类固醇特异性基因激活的关键机制.
结论:
- 细胞对外部刺激的反应可以通过表达单个转录因子来重新编程.
- 激素受体的差异表达是实现类固醇特异性基因激活的重要机制.
- 这项研究阐明了不同的类固醇激素如何通过共享的DNA结合部位选择性调节基因表达.
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