甲氧化酶:生物气体转化中的上游问题
Thomas J Lawton1, Amy C Rosenzweig1
1Departments of Molecular Biosciences and of Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
Journal of the American Chemical Society
|July 2, 2016
概括
由于缓慢的甲氧化酶,天然气生物转化为液体 (Bio-GTL) 面临挑战. 工程更快,更高效的酶是成功的生物燃料生产从甲至关重要.
科学领域:
- 生物技术
- 生物催化
- 生物化学工程
背景情况:
- 天然气生物转化为液体 (Bio-GTL) 提供了重要的经济潜力.
- 自然发生的甲氧化依赖于甲单氧酶 (MMO) 和甲基辅酶M减少酶 (MCR) 酶.
- 目前的酶是缓慢的,复杂的,很难为工业应用设计.
研究的目的:
- 评估甲氧化酶对生物GTL应用的适用性.
- 确定这些酶的工业用途的挑战和机遇.
- 评估不同甲氧化酶的优缺点.
主要方法:
- 对甲氧化酶的现有文献进行审查和分析.
- 酶动力学,效率和要求的比较
- 考虑基因操纵和重组的可操作性.
主要成果:
- 甲氧化酶 (MMO,MCR) 的周转率较低 (0.16-13s-1).
- 酶的表达,效率,辅助系统需求和复杂性各不相同.
- 为了提高酶的速度和简化, 工程的努力取得了有限的成功.
结论:
- 在Bio-GTL中需要显著提高酶速度和效率以实现高体积生产率.
- 对甲氧化酶的进一步研究对于开发可行的生物甲利用过程至关重要.
- 克服酶限制是释放Bio-GTL潜力的关键.
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