合成化合物的实时生物注释
Christopher J Gerry1, Bruce K Hua1, Mathias J Wawer
1Department of Chemistry and Chemical Biology, Harvard University , 12 Oxford Street, Cambridge, Massachusetts 02138, United States.
Journal of the American Chemical Society
|July 12, 2016
概括
研究人员开发了一种快速,低成本的方法,使用多重测试来测试新的化合物
科学领域:
- 化学生物学
- 药物化学
- 高含量查
背景情况:
- 有机化学家合成了许多复杂的分子.
- 大多数合成的化合物仍然未经生物活性测试.
- 测试往往发生太晚的合成规划.
研究的目的:
- 引入高维的多重测试,以快速,廉价地对新型化合物进行生物注释.
- 为了使生物分析能够用于高效的小分子图书馆建设.
- 通过整合实时生物反来指导合成工作.
主要方法:
- 通过光化学和热学重组合成十种构成异构化合物.
- 基于成像的"细胞绘画"试验的应用,以测量化合物诱导的细胞形态变化.
- 用于同时测量数千个细胞特征的高维多重量测试.
主要成果:
- 证明了对新合成化合物的实时生物注释的可行性.
- 确定了与细胞反应相关的化学结构的生物活动趋势.
- 展示多重测试如何为优化和图书馆设计提供信息.
结论:
- 通过多重测试进行实时生物注释可以加速药物发现和探测器开发.
- 这种方法有助于创建富含生物活性化合物的小分子库.
- 在合成初期整合生物反可以提高效率和结果.
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