针对神经元的光热激活的半导体聚合物纳米生物结合物
Yan Lyu1, Chen Xie1, Svetlana A Chechetka2
1School of Chemical and Biomedical Engineering, Nanyang Technological University , Singapore 637457, Singapore.
Journal of the American Chemical Society
|July 13, 2016
概括
研究人员开发了半导体聚合物纳米生物结合物 (SPNbc) 作为一种新的光遗传替代品. 这种光热方法使用近红外光来控制神经元活动而不需要基因修饰.
科学领域:
- 纳米技术和生物医学工程
- 神经科学和光遗传学
背景情况:
- 视觉遗传学提供精确的神经元控制, 但需要遗传修饰, 仅限于可见光波长.
- 现有的方法难以穿透深层组织, 阻碍了体内应用.
- 需要使用非侵入性,无基因的神经元控制方法.
研究的目的:
- 开发一种非遗传,近红外 (NIR) 光激活的神经元控制方法.
- 使用半导体聚合物纳米生物结合物 (SPNsbc) 作为光热纳米调节器.
- 研究SPNsbc在神经元中激活热敏离子通道的潜力.
主要方法:
- 合成的半导体聚合物纳米生物结合物 (SPNsbc) 在808nm处具有高NIR吸收.
- 与金纳米棒相比,证明了更高的光热转换效率.
- 使用SPNsbc来准和激活神经元中的热敏离子通道.
主要成果:
- 通过SPNsbc有效地将NIR光转化为热量,从而实现快速和局部的温度升高.
- 在神经细胞中实现了细胞内Ca2+) 流入的特异性和快速激活.
- 在没有基因转移的情况下, 证明了可逆和安全的神经元调节.
结论:
- SPNsbc为神经元控制提供了一个有希望的非遗传替代品.
- 这种光热策略可以使用NIR光透到深层组织.
- 基于有机纳米粒子的方法为远程细胞调节提供了多功能平台.
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