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化探针用于活细胞中的多色成像.

Gražvydas Lukinavičius1,2, Luc Reymond1, Keitaro Umezawa3

  • 1Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.

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|July 16, 2016
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概括

研究人员开发了一种新的远红色光体,SiR700,用于先进的活细胞成像. 这种-罗达胺染料能够实现多色超分辨率显微镜,增强细胞可视化能力.

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科学领域:

  • 细胞和分子成像技术
  • 生物光子学 生物光子学
  • 有机化学 有机化学

背景情况:

  • 活细胞成像需要具有特定光谱特性和与先进显微镜技术兼容性的光探针.
  • 现有的探测器可能在光谱范围或超分辨率应用的多色功能上有局限性.
  • -罗达胺染料具有适合生物成像的可调节光学特性.

研究的目的:

  • 为活细胞多色成像引入一种新型的深红色-罗达胺光体 (SiR700).
  • 开发和验证基于SiR700的探测器,用于各种蜂目标和应用.
  • 为了证明SiR700在多色活细胞超分辨率显微镜中的实用性.

主要方法:

  • 基于罗达胺的光SiR700.00的合成和表征.
  • 开发用于F-actin,微管,溶酶体和SNAP标签的SiR700结合探针.
  • 在活细胞中测试探头的性能,包括细胞透性,性和与超分辨率显微镜的兼容性.
  • 与基于SiR650的探测器一起进行多色成像实验.

主要成果:

  • SiR700在690nm和715nm分别表现出激发和辐射最大值.
  • 基于SiR700的探头是化,细胞透,适合超分辨率显微镜.
  • 多色活细胞超分辨率成像在远红光谱中使用SiR700和SiR650探针实现.
  • 新的探测器可以对细胞结构和过程进行增强的可视化.

结论:

  • SiR700是一种多功能远红色光体,用于先进的活细胞成像.
  • 基于SiR700的探针显著扩大了多色活细胞超分辨率显微镜的容量.
  • 这一发展为研究具有更高分辨率和光谱复杂化的动态细胞过程提供了新的可能性.