在真菌sesterpenoid生物合成中基于基因组发现了前所未有的循环模式
Masahiro Okada1, Yudai Matsuda1, Takaaki Mitsuhashi1
1Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan.
Journal of the American Chemical Society
|July 23, 2016
概括
研究人员使用真菌酶EvQS发现了一种新型的甲. 它的独特生物合成涉及复杂的重组,扩大了已知的类生物多样性.
科学领域:
- 自然产品化学
- 酵素学
- 生物合成
背景情况:
- 来自格兰酸盐 (GFPP) 的塞斯特类是具有多样性结构的罕见类.
- 由于对它们的生物合成途径的知识有限,许多sesterterpenoid支架仍然未被发现.
研究的目的:
- 为了描述真菌合成酶EvQS.
- 发现和阐明新型类的结构和生物合成.
主要方法:
- 基因组挖掘以识别潜在的合成酶.
- 使用EvQS与GFPP进行酶分析.
- 由此产生的塞斯特的结构阐明.
- 循环化过程的机制研究.
主要成果:
- 鉴定了真菌合成酶EvQS的特征.
- 获得了一种具有独特,拥挤的碳骨架的新型甲烯,甲烯 (1).
- 鉴定出氨酸 (2) 是氨酸的氧化产物.
- 基亚努拉的生物合成涉及前所未有的循环模式,具有多个化物转移和C-C键迁移.
结论:
- EvQS通过一种新的生物合成途径催化素的形成.
- 这一发现扩大了众所周知的类的结构多样性.
- 这些发现提供了有关生物合成演变的见解.
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