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人类SRMAtlas:定向测试的资源来量化完整的人类蛋白质组

Ulrike Kusebauch¹, David S Campbell¹, Eric W Deutsch¹

¹Institute for Systems Biology, Seattle, WA 98109, USA.

|July 26, 2016

概括

研究人员创建了一个含有超过166,000个测试的人类蛋白质图谱,用有针对性的质谱测量量几乎所有人类蛋白质. 这种资源有助于了解生物途径和疾病.

科学领域:

蛋白质组学
生物化学
系统生物学

背景情况:

了解人类蛋白质组对于解读细胞功能和疾病机制至关重要.
现有的蛋白质定量方法通常在范围,特异性或可访问性方面是有限的.

研究的目的:

开发一个用于量化几乎所有人类蛋白质的综合资源.
通过各种生物样本进行可靠和可重复的蛋白质测量.

主要方法:

创建和验证一个高度特定的测试汇编.
使用向质谱,专门选择反应监测 (SRM),以量化蛋白质.
开发了人类的SRMAtlas,为鉴定提供了最终的坐标.

主要成果:

对20277个注释的人类蛋白质中的99.7%进行量化测试.
数据包括166,174种蛋白型,提供每种蛋白质的多种测试.
测试可以识别拼接变体,突变和翻译后修改.
在研究细胞对胆固醇合成抑制和多赛治疗的有效性.

结论:

人类SRMAtlas是蛋白质组研究的宝贵,自由可用的资源.
能够对蛋白质进行全面的量化,促进系统生物学和疾病研究.
有助于更深入地了解细胞网络和治疗反应.

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