疫苗诱导的抗体可以中和1组和2组流感A病毒
M Gordon Joyce1, Adam K Wheatley1, Paul V Thomas1
1Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Cell
|July 26, 2016
概括
使用可以中和多种流感A病毒的抗体,可能实现通用流感疫苗. 疫苗接种增加了广泛的流感A中和B细胞,这表明了免疫策略的新指标.
科学领域:
- 免疫学
- 病毒学
- 疫苗学
背景情况:
- 开发一种通用流感疫苗仍然是一个重大挑战.
- 现有的疫苗针对特定的流感菌株,需要经常更新.
- 广泛中和的抗体为普遍流感保护提供了有前途的途径.
研究的目的:
- 识别和描述能够中和多种流感A病毒的抗体类别.
- 研究H5N1疫苗对大致中和B细胞的频率的影响.
- 提出指导通用流感免疫策略的指标.
主要方法:
- 从用H5N1DNA/MIV-prime-boost疫苗接种疫苗的受试者中对血凝素 (HA) 交叉反应的B细胞进行分类.
- 识别抗体类别并分析它们对各种流感A亚型的中和能力.
- 在B细胞群中确定特定抗体序列的频率.
- 用HA分析抗体的共晶结构以了解表位识别.
主要成果:
- 鉴定出了三种不同的抗体类别,可以中和多种1组和2组流感A病毒.
- 这些抗体类使用特征性生殖基因和聚合序列动机向HA干.
- 所有被分析的受试者拥有至少一个多捐赠者抗体的序列.
- 用不同的HA接种增加了编码大致中和抗体的B细胞的频率.
结论:
- 用不同类型的血凝素接种疫苗可以增加产生大致中和性流感A抗体的B细胞的流行率.
- 已识别的抗体类别及其对HA干表位的识别是广泛中和的关键.
- 量化这些多捐赠者B细胞序列的患病率可以作为优化通用流感A疫苗策略的指标.
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