Search research articles

关于 JoVE

概览领导团队博客 JoVE 帮助中心

作者

出版流程编辑委员会范围与政策同行评审常见问题投稿

图书馆员

用户评价订阅访问资源图书馆顾问委员会常见问题

研究

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments 存档

教育

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual 教师资源中心教师网站

使用条款与条件

相关概念视频

Microbial Biosensors

Microbial Biosensors

Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序

Same author

Beyond component optimization: systems-level biodesign for lanthanide recovery.

Current opinion in biotechnology·2026

Same author

Aesthetic techniques for melanocytic nevus management: clinical outcomes, cosmetic satisfaction, and safety-a systematic review and meta-analysis.

Frontiers in medicine·2026

Same author

Water-Soluble Viscosity-Activated Molecular Rotor for <i>In Situ</i> Imaging of Liver Damage in the NIR-II Window.

ACS sensors·2026

Same author

Conflicting Roles of Urotensin II in Chronic Kidney Disease: Insights from Clinical and Mechanistic Studies.

Journal of the College of Physicians and Surgeons--Pakistan : JCPSP·2026

Same author

DNA Scission by Non-Histidine Amino-Terminal Cu(II) and Ni(II) Binding-Like Peptides.

Chembiochem : a European journal of chemical biology·2026

Same author

Bioactive co-assembly of PSA/diT-VES nanomicelles orchestrates macrophage reprogramming for acute lung injury therapy.

Drug delivery and translational research·2026

Search research articles

相关实验视频

Updated: May 4, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

作为通用生物传感器平台的工程PQQ-葡萄糖脱酶

Zhong Guo¹, Lindy Murphy², Viktor Stein¹

¹Institute for Molecular Bioscience and Australian Institute for Bioengineering and Nanotechnology, The University of Queensland , Brisbane, QLD 4072, Australia.

Journal of the American Chemical Society

|July 28, 2016

概括

这项研究引入了一种新的生物传感器设计,使用重新设计的葡萄糖脱酶来检测各种分析物. 这种多功能生物传感器架构允许稳定,干燥的存储和直接的电子监控,扩大诊断能力.

更多相关视频

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

相关实验视频

Last Updated: May 4, 2026

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis

Published on: April 11, 2016

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

Published on: August 8, 2016

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Design to Implementation Study for Development and Patient Validation of Paper-Based Toehold Switch Diagnostics

Published on: June 17, 2022

科学领域:

生物技术和生物传感器开发
酶工程和复制
电化学传感平台

背景情况:

现有的直接电子输出生物传感器受到自然存在的酶的限制,限制了分析物的检测范围.
需要可适应的生物传感器平台,与便携式电子设备和各种分析剂兼容.

研究的目的:

基于分析剂驱动的分子间重组和活性复合的新型生物传感器架构.
展示这种架构的适应性,用于检测各种目标,包括药物和蛋白质活动.
使生物传感器活动的直接电子监控和长期存储成为可能.

主要方法:

重新设计PQQ-葡萄糖脱酶以进行分析驱动的活性复制.
开发一种新的生物传感器架构,促进分子间重组.
连接剂诱导的生物传感器活动的时空测量监测
测试检测免疫抑制药物,α-氨基酶,血栓和XA因子的传感器性能.

主要成果:

在新型生物传感器架构中成功展示了分析剂驱动的活性复合.
快速采用检测各种分析物的传感器:免疫抑制药物,α-amylase,血栓和XA因子.
生物传感器在干燥状态下表现出稳定性,没有显著的活性损失.
直接的时空测量使得一次性传感电极的制造成为可能.

结论:

这种生物传感器架构为检测广泛的分析物提供了多功能和适应性平台.
生物传感器的稳定性和直接电子读数有助于实际应用,包括一次性传感器.
这种架构具有扩展潜力,可以检测各种生物分子,核酸和无机化合物.