单个大肠杆菌细胞的复制和分裂周期的同步
Mats Wallden1, David Fange1, Ebba Gregorsson Lundius1
1Department of Cell and Molecular Biology, Science for Life Laboratory, Uppsala University, Husargatan 3, 75124 Uppsala, Sweden.
Cell
|July 30, 2016
概括
大肠杆菌生长的细胞变异性是由染色体复制启动的固定体积触发因素解释的. 这种机制驱动了"虫"的行为,细胞每代增加一个一致的尺寸增量.
科学领域:
- 微生物学
- 细胞生物学
- 系统生物学
背景情况:
- 即使在恒定的环境条件下,同源性大肠杆菌 (大肠杆菌) 种群在生长速度,分裂大小和生成时间方面表现出显著的细胞间变异性.
- 一个关键的观察是
- 一个毒蛇
- 细胞在每一代中增加与出生大小无关的尺寸.
研究的目的:
- 研究大肠杆菌的潜在机制
- 一个毒蛇
- 他们的行为.
- 绘制染色体复制和细胞分裂周期之间的关系.
主要方法:
- 使用光显微镜监测单个大肠杆菌细胞.
- 在实时跟踪染色体复制和细胞分裂事件.
主要成果:
- 染色体复制的启动是在每个染色体的固定细胞体积下触发的,不论出生体积或生长速度如何.
- 每个复制启动事件都与随后的细胞分裂事件联系在一起,时间持续时间取决于生长速度.
- 细胞间的分裂时间和细胞大小的变化主要是由生长速度的波动驱动的.
结论:
- 这项研究为观察到的大肠杆菌提供了一种机制性的解释.
- 一个毒蛇
- 他们的行为.
- 该模型解释了为什么快速生长的细胞会表现出增长的行为,并预测了较慢的生长速度的偏差.
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