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Updated: Mar 17, 2026

Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
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可逆的寡核酸链阻断使T细胞受体α和β链mRNA的捕获和放大成为可能

W Miachel Hanson, Zhe Chen, Laurie K Jackson

  • 1Division of Infection & Immunity, Cardiff University School of Medicine , Cardiff, United Kingdom.

Journal of the American Chemical Society
|August 2, 2016
PubMed
概括

研究人员开发了能够从单个细胞中捕获多个mRNA序列的新型DNA条形码珠. 这种进步增强了基因分析的应用,

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Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
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Although all next-generation methods use different technologies, they all share a set of standard features....
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...
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