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相关概念视频

Protein Complex Assembly02:41

Protein Complex Assembly

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Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
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相关实验视频

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ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
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ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

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表面修饰蛋白质的纳米粒子组合

Matthias Fach1, Lydia Radi1, Peter R Wich1

  • 1Institut für Pharmazie und Biochemie, Johannes Gutenberg-Universität Mainz , Staudingerweg 5, 55128 Mainz, Germany.

Journal of the American Chemical Society
|August 5, 2016
PubMed
概括

研究人员使用简单的纳米乳液技术创建了基于蛋白质的新型纳米颗粒. 这些稳定的100纳米粒子由表面修饰的蛋白质制成,如酶,有效地将多克索鲁比辛输入癌细胞.

科学领域:

  • 生物材料科学
  • 纳米技术
  • 药物输送系统

背景情况:

  • 蛋白质和是大自然中高度定义的大分子.
  • 开发稳定,多功能纳米粒子载体对于有效的药物输送至关重要.

研究的目的:

  • 开发使用表面修饰蛋白质的新型纳米颗粒药物输送系统.
  • 通过简单的纳米乳液技术展示基于蛋白质的纳米颗粒的制备.
  • 评估这些纳米粒子的药物加载和释放能力.

主要方法:

  • 通过高表面PEGylation对蛋白质进行表面修饰,以使其在有机溶剂中溶解.
  • 在没有变性或交叉连接的情况下,用于纳米粒子形成的油与水纳米乳液技术.
  • 准备空的和装有多克索鲁的酶纳米颗粒 (大约 它们的直径为100nm.

主要成果:

  • 通过PEGylation实现蛋白质可溶性切换,同时保持原生蛋白质结构.
  • 使用简单的纳米乳液方法制造出稳定的基于蛋白质的纳米粒子 (100nm).
  • 在细胞吸收后,已证明多克索鲁比辛在癌细胞中的释放时间依赖.

结论:

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Rapid, Scalable Assembly and Loading of Bioactive Proteins and Immunostimulants into Diverse Synthetic Nanocarriers Via Flash Nanoprecipitation
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  • 开发的方法为制造基于蛋白质的纳米粒子提供了通用方法.
  • 表面修饰的蛋白质可以作为稳定的纳米颗粒药物载体的结构材料.
  • 这项技术对使用生物材料的先进治疗系统具有前景.