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Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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使用杂交的原生生物和脊椎动物适应性免疫系统进行向核酸编辑

Keiji Nishida1, Takayuki Arazoe1, Nozomu Yachie2

  • 1Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai-cho, Nada-ku, Kobe, Hyogo 657-8501, Japan.

Science (New York, N.Y.)
|August 6, 2016
PubMed
概括

研究人员设计了一种合成复合物,名为Target-AID,它结合了CRISPR/Cas9和脱氨酶,用于精确的DNA单核酸替代. 这种方法降低了毒性,并有效地诱导基因材料的向点突变.

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