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Updated: Mar 16, 2026

A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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酸:一种生物灵感的策略,提高光NADH探测器的灵敏度和选择性

Lu Wang1, Jingye Zhang2, Beomsue Kim3

  • 1Department of Chemistry and Medicinal Chemistry Program, National University of Singapore , Singapore 117543.

Journal of the American Chemical Society
|August 9, 2016
PubMed
概括

研究人员开发了一种新的光探针,用于检测降低的尼古丁胺氨基二核酸 (NADH). 这种敏感和选择性的探针在没有酶的情况下工作,可以测量医疗保健产品和活细胞中的NADH.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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