来自Methylosinus sp的甲诺巴克的表征 没有
Grace E Kenney1, Anthony W Goering1, Matthew O Ross1
1Departments of †Molecular Biosciences and ‡Chemistry, Northwestern University , Evanston, Illinois 60208, United States.
Journal of the American Chemical Society
|August 17, 2016
概括
研究人员从Methylosinus sp中特征化了甲巴 (Mbns). 显示出一个明显的脊柱. 这项研究验证了基因组挖掘用于发现新型MBN和理解它们的生物合成.
科学领域:
- 自然产品化学
- 微生物学
- 基因组学
背景情况:
- 甲巴 (Mbns) 是天然产品,以铜结合而闻名,具有独特的异环和胺.
- 基因组挖掘已经确定了Mbn操作子,从而能够预测多样化的Mbn结构.
- 了解Mbn多样性和生物合成对于发现天然产品至关重要.
研究的目的:
- 描述来自Methylosinus (Ms.) sp的甲巴. 在LW4.
- 将此 Mbn 的结构与之前识别的 Mbn 进行比较.
- 验证基因组分析用于预测Mbn结构和生物合成途径.
主要方法:
- Mbn与 Ms. sp 的分离和结构特征 在LW4.
- 分析 Ms. sp 的情况. 一个LW4 Mbn的操作.
- 标签: Ms. sp 的比较 已知 Mbns 的 LW4 Mbn 序列和修改.
主要成果:
- 这位女士的MBN LW4具有独特的骨,与其他已知的Mbns不同.
- 观察到的翻译后变化与基于 Ms. sp 的预测一致. 一个LW4 Mbn的操作.
- 通过实验性表征证实了基于基因组的Mbn结构预测.
结论:
- 基因组挖掘与结构阐明相结合是一种有效的基因组学方法,用于发现多样化的Mbns.
- 这项研究扩大了已知的甲巴的结构多样性.
- 这些发现为甲巴的生物合成途径提供了洞察力.
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