纳米纤维的细胞环境差异化自组装
Zhen Zheng1, Peiyao Chen1, Maolin Xie1
1CAS Key Laboratory of Soft Matter Chemistry, †Department of Chemistry and ⊥Department of Polymer Science and Engineering and ‡Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China , 96 Jinzhai Road, Hefei, Anhui 230026, China.
Journal of the American Chemical Society
|August 18, 2016
概括
这项研究引入了一种新型的前体,它可以在癌细胞内自组合成两种不同的纳米纤维,从而区分细胞外和细胞内环境,用于特定的生物应用.
科学领域:
- 生物材料科学
- 超分子化学
- 纳米技术
背景情况:
- 细胞环境为超分子自组提供了独特的机会.
- 从单一的前体中区分细胞外和细胞内环境形成不同的纳米纤维仍然是一个重大挑战.
研究的目的:
- 设计一种能够在不同的细胞环境 (细胞外和细胞内) 中形成两种不同的纳米纤维的前体.
- 为了利用性酸酶 (ALP) 和谷氨 (GSH) 在癌细胞中的差异性存在来进行序列自组.
- 通过点击化学建立超分子纳米纤维容易后修改的平台.
主要方法:
- 合理设计的前体分子 (Cys(SEt) -Glu-Tyr ((H2PO3) -Phe-Phe-Gly-CBT) 结合了对ALP和GSH敏感的可裂变链接.
- 用ALP和GSH对前体进行序列处理,以诱导分化和自组合成两性纳米纤维.
- 使用点击凝结反应进行组装后的修改.
主要成果:
- 在顺序的酶和化学处理后,设计的前体成功产生了两个不同的两结构.
- 这些结构在水凝环境中自组装成不同的纳米纤维,证明了对环境的响应性.
- 这种点击凝结反应使得形成的纳米纤维容易后调节.
结论:
- 一种多功能前体被开发出来,用于两个不同的纳米纤维的顺序性,环境特定的自组装.
- 这种方法为向癌细胞内的细胞外和细胞内环境提供了一种新的策略.
- 该平台为先进的生物应用和药物输送系统提供了潜力.
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