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抗体介导的SHIV挑战保护包括系统清除远端病毒

Jinyan Liu¹, Khader Ghneim², Devin Sok³

¹Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02215, USA.

Science (New York, N.Y.)

|August 20, 2016

概括

广泛中和抗体 (bNAbs) 通过清除远处组织中的病毒来保护子免受SHIV感染,从而揭示了对粘膜病毒挑战的新保护机制.

科学领域:

免疫学
病毒学
灵长类动物

背景情况:

广泛中和抗体 (bNAbs) 在预防HIV-1感染方面具有前景.
对粘膜病毒挑战的bNAb介导保护的确切机制和部位尚不完全理解.

研究的目的:

在 rhesus 模型中调查抗体拦截的位置和bNAbs 对人类免疫缺陷病毒 (SHIV) 的保护机制.

主要方法:

给 rhesus 子使用 bNAb PGT121 的保护剂量.
用SHIV-SF162P3进行阴道内挑战.
在距离组织中检测病毒RNA和DNA.
病毒RNA阳性组织的转录组分析.
通过采用转移评估这些组织细胞的传染性.

主要成果:

在接受PGT121治疗的动物的远端组织中检测到低水平的病毒RNA和DNA在挑战后的7天内.
病毒RNA阳性组织表现出先天免疫激活的转录基因特征.
这些组织的细胞能够在转移到原始宿主时引发感染,这表明残留的传染性病毒.

结论:

通过bNAb对粘膜SHIV的保护包括从远端组织清除感染性病毒.
这项研究揭示了抗体介导的新型保护机制,突出显示了远端组织病毒清除在预防感染中的作用.

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